Wound Hormones of Plants H. the Isolation of a Crystalline Active Substance
نویسنده
چکیده
abalone sperm appears simply to be concerned in the penetration part of the fertilization process. Summary.-A lytic agent having the property of dissolving the egg membrane has been extracted from sperm of the giant keyhole limpet (Megathura crenulata). The lysin is non-dialyzable and readily inactivated by heat. It precipitates upon saturation of the extract with ammonium sulphate. The precipitate gives the usual protein tests and pure proteinases inactivate the lytic agent. Abalone (Haliotis cracherodii) sperm also yield a lysin which acts on the eggs of the same species. Cross-lysis between limpet and abalone does not occur; nor does cross-fertilization. Limpet sperm extracts also contain a substance (anti-fertilizin) capable of neutralizing the sperm agglutinin (fertilizin) obtained from eggs. Heat inactivation of the lysin leaves the anti-fertilizin unaffected.
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